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Amersham Life Sciences Inc multiphor ii apparatus
Specification of the Relevant Instrumental Techniques for LF Characterization
Multiphor Ii Apparatus, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiphor+ii+apparatus/multiphor+ii+system/pmc10755757-12-50-53
Average 90 stars, based on 1 article reviews
multiphor ii apparatus - by Bioz Stars, 2026-10
90/100 stars

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1) Product Images from "The Multifaceted Roles of Bovine Lactoferrin: Molecular Structure, Isolation Methods, Analytical Characteristics, and Biological Properties"

Article Title: The Multifaceted Roles of Bovine Lactoferrin: Molecular Structure, Isolation Methods, Analytical Characteristics, and Biological Properties

Journal: Journal of Agricultural and Food Chemistry

doi: 10.1021/acs.jafc.3c06887

Specification of the Relevant Instrumental Techniques for LF Characterization
Figure Legend Snippet: Specification of the Relevant Instrumental Techniques for LF Characterization

Techniques Used: Isolation, Glycoproteomics, Staining, Mass Spectrometry, Concentration Assay, Spectrophotometry, Clinical Proteomics, Zeta Potential Analyzer, Marker, Spectroscopy, Software, Raman Spectroscopy, Functional Assay, Fluorescence, Sequencing, Single Particle, Microscopy, Chromatography, Solvent, Evaporation, Differential Scanning Calorimetry, Injection

Related Articles

Stripping Membranes:

Article Title: Targets downstream of Cdk8 in Dictyostelium development
Article Snippet: .. The samples were absorbed into a nonlinear immobilized pH gradient strip (pH 3-11, Amersham) and isoelectric focusing performed using MULTIPHOR II apparatus (Amersham) according to the manufacturer’s guidelines [39]. ..

Article Title: Targets downstream of Cdk8 in Dictyostelium development
Article Snippet: .. The samples were absorbed into a nonlinear immobilized pH gradient strip (pH 3-11, Amersham) and isoelectric focusing performed using MULTIPHOR II apparatus (Amersham) according to the manufacturer's guidelines [ ]. ..

Membrane:

Article Title: Agaricus bisporus and related Agaricus species on lignocellulose: production of manganese peroxidase and multicopper oxidases.
Article Snippet: 1087-1845/$ see front matter 2013 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.fgb.2013.02.002 ⇑ Corresponding author.. Address: Department of Food and Environmental Sciences, Biocenter 1, P.O.. Box 56, FI-00014 University of Helsinki, Finland.

Electrofocusing:

Article Title: Agaricus bisporus and related Agaricus species on lignocellulose: production of manganese peroxidase and multicopper oxidases.
Article Snippet: 1087-1845/$ see front matter 2013 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.fgb.2013.02.002 ⇑ Corresponding author.. Address: Department of Food and Environmental Sciences, Biocenter 1, P.O.. Box 56, FI-00014 University of Helsinki, Finland.

Article Title: Differential regulation of manganese peroxidases and characterization of two variable MnP encoding genes in the white-rot fungus Physisporinus rivulosus.
Article Snippet: Manganese peroxidase (MnP) production in the white-rot basidiomycete Physisporinus rivulosus T241i was studied.. Separate MnP isoforms were produced in carbonlimited liquid media supplemented with Mn, veratryl alcohol, or sawdust.. The isoforms had different pH ranges for the oxidation of Mn and 2,6-dimethoxyphenol.

Article Title: The Multifaceted Roles of Bovine Lactoferrin: Molecular Structure, Isolation Methods, Analytical Characteristics, and Biological Properties
Article Snippet: particle charge properties , zeta potential measurements , spectroscopic , Electrophoretic mobility was determined based on the Henry equation. The zeta potential was calculated according to the Smoluchowski approximation. The measured zeta potential is highly influenced by the type of electrolyte (NaCl, KCl) used and ionic strength. , Malvern Nano-ZS ZetaSizer (Malvern Instru ments, U.K.); temperature, 25 °C; cuvette, DTS1070; equilibration time, 2 min , ( , ) . .. , IEF , electromigration , Different pI values determined by IEF also might be a matter of the selected separation conditions, e.g. the type of carrier ampholyte (CA), pI marker, buffer composition, and separation time. The possibility of distinction of several LF fractions is based on their pI values. , Multiphor II apparatus (Amersham, Freiburg, Germany); precast gels Servalyt and Blank precotes (Serva, Germany); gel parameters, 126 × 125 × 0.3 mm; temperature, 5–8 °C; voltage, 2000 V; current, 6 mA; power, 12 W , ( , ) . ..

Article Title: Transcriptome and Proteome Expressions Involved in Insulin Resistance in Muscle and Activated T-Lymphocytes of Patients with Type 2 Diabetes
Article Snippet: .. A Multiphor II apparatus (Amersham) for IEF and a Protean Dodeca Plus apparatus (Bio-Rad, Hercules, USA) for SDS-PAGE were used in this study as previously describled , . ..

other:

Article Title: Effect of copper, nutrient nitrogen, and wood-supplement on the production of lignin-modifying enzymes by the white-rot fungus Phlebia radiata.
Article Snippet: Production of the oxidoreductive lignin-modifying enzymes e lignin and manganese peroxidases (MnPs), and laccase e of the white-rot basidiomycete Phlebia radiata was investigated in semi-solid cultures supplemented with milled grey alder or Norway spruce and charcoal.. Concentrations of nutrient nitrogen and Cu-supplement varied also in the cultures.. According to extracellular activities, production of both lignin peroxidase (LiP) and MnP was significantly promoted with wood as carbon source, with milled alder (MA) and low nitrogen (LN) resulting with the maximal LiP activities (550 nkat l ) and noticeable levels of MnP (3 mkat l ).

Polyacrylamide Gel Electrophoresis:

Article Title: Impact of the iron saturation of bovine lactoferrin on adsorption to a strong cation exchanger membrane
Article Snippet: Bovine lactoferrin (bLf) is a high-value iron-binding whey protein used for infant nutrition because of several health-promoting activities.. The bLf concentration in whey is very low.. Most methods for Lf enrichment use ion exchange chromatography since it is highly selective.

Marker:

Article Title: The Multifaceted Roles of Bovine Lactoferrin: Molecular Structure, Isolation Methods, Analytical Characteristics, and Biological Properties
Article Snippet: particle charge properties , zeta potential measurements , spectroscopic , Electrophoretic mobility was determined based on the Henry equation. The zeta potential was calculated according to the Smoluchowski approximation. The measured zeta potential is highly influenced by the type of electrolyte (NaCl, KCl) used and ionic strength. , Malvern Nano-ZS ZetaSizer (Malvern Instru ments, U.K.); temperature, 25 °C; cuvette, DTS1070; equilibration time, 2 min , ( , ) . .. , IEF , electromigration , Different pI values determined by IEF also might be a matter of the selected separation conditions, e.g. the type of carrier ampholyte (CA), pI marker, buffer composition, and separation time. The possibility of distinction of several LF fractions is based on their pI values. , Multiphor II apparatus (Amersham, Freiburg, Germany); precast gels Servalyt and Blank precotes (Serva, Germany); gel parameters, 126 × 125 × 0.3 mm; temperature, 5–8 °C; voltage, 2000 V; current, 6 mA; power, 12 W , ( , ) . ..

SDS Page:

Article Title: Transcriptome and Proteome Expressions Involved in Insulin Resistance in Muscle and Activated T-Lymphocytes of Patients with Type 2 Diabetes
Article Snippet: .. A Multiphor II apparatus (Amersham) for IEF and a Protean Dodeca Plus apparatus (Bio-Rad, Hercules, USA) for SDS-PAGE were used in this study as previously describled , . ..



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Specification of the Relevant Instrumental Techniques for LF Characterization

Journal: Journal of Agricultural and Food Chemistry

Article Title: The Multifaceted Roles of Bovine Lactoferrin: Molecular Structure, Isolation Methods, Analytical Characteristics, and Biological Properties

doi: 10.1021/acs.jafc.3c06887

Figure Lengend Snippet: Specification of the Relevant Instrumental Techniques for LF Characterization

Article Snippet: , IEF , electromigration , Different pI values determined by IEF also might be a matter of the selected separation conditions, e.g. the type of carrier ampholyte (CA), pI marker, buffer composition, and separation time. The possibility of distinction of several LF fractions is based on their pI values. , Multiphor II apparatus (Amersham, Freiburg, Germany); precast gels Servalyt and Blank precotes (Serva, Germany); gel parameters, 126 × 125 × 0.3 mm; temperature, 5–8 °C; voltage, 2000 V; current, 6 mA; power, 12 W , ( , ) .

Techniques: Isolation, Glycoproteomics, Staining, Mass Spectrometry, Concentration Assay, Spectrophotometry, Clinical Proteomics, Zeta Potential Analyzer, Marker, Spectroscopy, Software, Raman Spectroscopy, Functional Assay, Fluorescence, Sequencing, Single Particle, Microscopy, Chromatography, Solvent, Evaporation, Differential Scanning Calorimetry, Injection